Rat cells and I
In continuing the stream of scary pictures on the blog, I introduce to you the subject of my recurring nightmares, the source of my misery, the cause of my depression, the reason behind my suicide:
Blog, PC12 cells. PC12, Blog.
Sure they are small. And look fairly harmless. But I assure you, they can give torture like no other.
Much to my supervisor's dismay, I still don't know very much about these cells. But I shall tell you what I know about them here. They are tumorous rat neurons that are terribly vulnerable to infection attacks (a major potential hazard in my way to a degree) and they love to clump together which annoys my supervisor thus another threat to my graduation. Normally, they should look like the roundish blobs you see above (actually, they should be "irregularly shaped" but whatever). If you feed to them this expensive chemical like I do called NGF, or Nerve Growth Factor, however, they grow long protrusions like what you see in the centre of the picture.
Or they are supposed to. Obviously they are doing anything but that in my experiment.
On the plate where I grow my cells and feed them NGF, instead of differenciating (i.e. growing their processes), they are multiplying (something they are NOT supposed to do because I am not feeding them anything to encourage proliferation), quickly filling up the area and making the plate to look like the "surface of Mars", according to a random passer by who was probably disappointed when I told him that was no Mars he was seeing.
Since this is the debut appearance of PC12 on the blog, I shall say only nice things about it.
...
Ok I give up. I have nothing more to say.
2 Comments:
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grow some damn processes already damn rat cells! If you don't, I'll lock you in a room with Michael Jackson!
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